Hotspot

psbM-trnD-GUC

LSC · 31373-32544 · 1172 bp

Back to Hedera

Pi 0.00156
MarkerSeek score 49.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1172
variable_sites
3
pi
0.00156
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGCAGAAAGGCAGTAGGAAC TTCAATTGGTCAGAGCACCG 50.0 50.0 57.5 58.5 1304 bp 1.0 72.5
psbM-trnD-GUC_p2 GCAGAAAGGCAGTAGGAACT TTCAATTGGTCAGAGCACCG 50.0 50.0 57.5 58.5 1303 bp 1.0 72.5
psbM-trnD-GUC_p3 AGCAGAAAGGCAGTAGGAAC GTTCAATTGGTCAGAGCACC 50.0 50.0 57.5 57.0 1305 bp 1.0 68.4
psbM-trnD-GUC_p4 GCAGAAAGGCAGTAGGAACT GTTCAATTGGTCAGAGCACC 50.0 50.0 57.5 57.0 1304 bp 1.0 68.4
psbM-trnD-GUC_p5 AAGCAGAAAGGCAGTAGGAAC TTCAATTGGTCAGAGCACCG 47.6 50.0 58.2 58.5 1305 bp 1.0 66.6