Hotspot

trnK-UUU-rps16

LSC · 4332-5229 · 898 bp

Back to Hedera

Pi 0.00223
MarkerSeek score 51.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
898
variable_sites
4
pi
0.00223
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCTTCGCTTCATCCA TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.1 58.3 1050-1084 bp 1.0 77.5
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1063-1097 bp 1.0 77.3
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 1002-1036 bp 1.0 76.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 1001-1035 bp 1.0 76.6
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1087-1121 bp 1.0 76.1