Hotspot

ndhF

SSC · 1-651 · 651 bp

Back to Hechtia

Pi 0.012

MarkerSeek score 59.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 651
variable_sites: 30
pi: 0.012
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AACCAAAATCCCCTACACGA	TTTTAATACTTCTATGCTGGGA	45.0	31.8	56.7	52.6	172-176 bp	0.857143	41.1
ndhF_p2	TCGAGCAAATCCCGAAATTCT	TTTTAATACTTCTATGCTGGGA	42.9	31.8	58.0	52.6	236-240 bp	0.857143	39.3
ndhF_p3	TCGAGCAAATCCCGAAATTCT	TTTTTAATACTTCTATGCTGGGA	42.9	30.4	58.0	53.4	237-241 bp	0.857143	39.3
ndhF_p4	TCGAGCAAATCCCGAAATTCT	TTAATACTTCTATGCTGGGATT	42.9	31.8	58.0	52.4	234-238 bp	0.857143	39.3
ndhF_p5	TCGAGCAAATCCCGAAATTCT	TTTAATACTTCTATGCTGGGAT	42.9	31.8	58.0	52.4	235-239 bp	0.857143	39.3