Hotspot

petA-psbJ

LSC · 107855-108981 · 1127 bp

Back to Hechtia

Pi 0.0188

MarkerSeek score 77.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1127
variable_sites: 57
pi: 0.0188
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.7	58.0	1240-1276 bp	1.0	80.2
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTCT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.7	58.0	1238-1274 bp	1.0	80.2
petA-psbJ_p3	GATCCATTACGCGTCCAAGG	TCTGGGTTGGGTTCATCTCT	55.0	50.0	58.8	58.0	1251-1287 bp	1.0	79.4
petA-psbJ_p4	GATCCATTACGCGTCCAAGG	TGGGTTGGGTTCATCTCTCT	55.0	50.0	58.8	58.0	1249-1285 bp	1.0	79.4
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	CTGGGTTGGGTTCATCTCTC	50.0	55.0	58.7	57.6	1239-1275 bp	1.0	77.3