Hotspot

accD-psaI

LSC · 103287-104133 · 847 bp

Back to Hechtia

Pi 0.00843

MarkerSeek score 70.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 36
pi: 0.00843
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	781-1059 bp	1.0	85.0
accD-psaI_p2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	780-1058 bp	1.0	85.0
accD-psaI_p3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	787-1065 bp	1.0	84.3
accD-psaI_p4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	786-1064 bp	1.0	84.3
accD-psaI_p5	GCTCCATGGTTTCTTTCCCT	ATTGCCGGAAATACTAGGGC	50.0	50.0	57.8	57.7	772-1050 bp	1.0	83.0