Hotspot

rps4-trnT-UGU

LSC · 91781-92122 · 342 bp

Back to Hechtia

Pi 0.0101

MarkerSeek score 48.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 12
pi: 0.0101
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGACGGCGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	58.3	58.2	415-444 bp	1.0	80.9
rps4-trnT-UGU_p2	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	446-475 bp	1.0	80.8
rps4-trnT-UGU_p3	TCCCAGAACGCAATTGGTTT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	58.3	57.9	515-544 bp	1.0	80.0
rps4-trnT-UGU_p4	TTCCCAGAACGCAATTGGTT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	58.3	57.9	516-545 bp	1.0	80.0
rps4-trnT-UGU_p5	TTTCCCAGAACGCAATTGGT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	58.3	57.9	517-546 bp	1.0	80.0