Hotspot

psaA-ycf3

LSC · 87532-88199 · 668 bp

Back to Hechtia

Pi 0.00872

MarkerSeek score 62.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 26
pi: 0.00872
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	GTTGAAAATCACGAAGCGCT	50.0	45.0	57.9	57.7	758-777 bp	1.0	81.8
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	802-821 bp	1.0	81.2
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	CCGATCAAGCTGCTGAGTAT	50.0	50.0	57.9	57.5	831-850 bp	1.0	79.7
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TCCGATCAAGCTGCTGAGTA	50.0	50.0	57.9	58.5	832-851 bp	1.0	79.7
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGAAAATCACGAAGCGCTTC	50.0	45.0	57.9	57.4	756-775 bp	1.0	79.6