Hotspot

rps16-trnQ-UUG

LSC · 49554-51059 · 1506 bp

Back to Hechtia

Pi 0.00584
MarkerSeek score 68.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1506
variable_sites
43
pi
0.00584
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAAATCAAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1612-1677 bp 1.0 85.8
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAAATCAAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1610-1675 bp 1.0 85.8
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAAATCAAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1618-1683 bp 1.0 85.7
rps16-trnQ-UUG_p4 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1554-1619 bp 1.0 85.3
rps16-trnQ-UUG_p5 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1552-1617 bp 1.0 85.3