Hotspot

rpl32-trnL-UAG

SSC · 1904-2693 · 790 bp

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Pi 0.00977

MarkerSeek score 69.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 790
variable_sites: 33
pi: 0.00977
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CACCGGGCATTCAAAAAGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.5	58.0	847-878 bp	1.0	82.2
rpl32-trnL-UAG_p2	CACCGGGCATTCAAAAAGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.5	58.0	848-879 bp	1.0	82.2
rpl32-trnL-UAG_p3	CACCGGGCATTCAAAAAGTT	TTTAGAAGATGCCGCCACTC	45.0	50.0	57.5	58.0	907-938 bp	1.0	81.7
rpl32-trnL-UAG_p4	TTCCACCGGGCATTCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	850-881 bp	1.0	81.7
rpl32-trnL-UAG_p5	TTTCCACCGGGCATTCAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	851-882 bp	1.0	81.6