Hotspot

rps2

LSC · 53779-54468 · 690 bp

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Pi 0.0698
MarkerSeek score 80.70
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
LSC
length_bp
690
variable_sites
71
pi
0.0698
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 AACAAGAGAAACAGCCTGCA AGTTATCTGTTTCCAAGCGGT 45.0 42.9 57.9 57.6 1468 bp 0.333333 41.5
rps2_p2 AACAAGAGAAACAGCCTGCA ACAACCCATTTTGCTTTTCGT 45.0 38.1 57.9 57.5 1266 bp 0.333333 40.6
rps2_p3 AAGAGAAACAGCCTGCATGT AGTTATCTGTTTCCAAGCGGT 45.0 42.9 57.7 57.6 1465 bp 0.333333 39.7
rps2_p4 ACAAGAGAAACAGCCTGCAT AGTTATCTGTTTCCAAGCGGT 45.0 42.9 57.7 57.6 1467 bp 0.333333 39.7
rps2_p5 AGAGAAACAGCCTGCATGTT AGTTATCTGTTTCCAAGCGGT 45.0 42.9 57.7 57.6 1464 bp 0.333333 39.7