Hotspot

rbcR

SSC · 112753-113676 · 924 bp

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Pi 0.0801
MarkerSeek score 81.10
Primer available yes
Label rbcR

Feature Metadata

label_name
rbcR
feature_type
gene
region
SSC
length_bp
924
variable_sites
107
pi
0.0801
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcR_p1 TGTCCTTGTTTCTTGCGTGT TGCTGCTTCATGGGCATTAT 45.0 45.0 58.3 57.9 1780-1811 bp 0.666667 70.9
rbcR_p2 TGTCCTTGTTTCTTGCGTGT TAATTGCTGCTTCATGGGCA 45.0 45.0 58.3 58.2 1784-1815 bp 0.666667 70.3
rbcR_p3 TGTCCTTGTTTCTTGCGTGT TTGCTGCTTCATGGGCATTA 45.0 45.0 58.3 58.2 1781-1812 bp 0.666667 70.3
rbcR_p4 TGTCCTTGTTTCTTGCGTGT GGAGTTCAAGCAGCTCAGAA 45.0 50.0 58.3 57.8 1565 bp 0.333333 49.9
rbcR_p5 TGTCCTTGTTTCTTGCGTGT AAGGAGTTCAAGCAGCTCAG 45.0 50.0 58.3 57.8 1567 bp 0.333333 49.8