Hotspot

psaF

LSC · 9767-10324 · 558 bp

Pi 0.0717

MarkerSeek score 80.40

Primer available yes

Label psaF

Feature Metadata

label_name: psaF
feature_type: gene
region: LSC
length_bp: 558
variable_sites: 58
pi: 0.0717
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaF_p1	ATTGTCCAAAATGCGCACTG	GGGCAAGTTAGCGTCTGAAT	45.0	50.0	57.9	58.3	1876 bp	0.333333	50.7
psaF_p2	GTCCAAAATGCGCACTGAAA	GGGCAAGTTAGCGTCTGAAT	45.0	50.0	57.9	58.3	1873 bp	0.333333	50.2
psaF_p3	GTTGAGCCCATTTCGCAAAA	GGGCAAGTTAGCGTCTGAAT	45.0	50.0	57.9	58.3	1729 bp	0.333333	50.1
psaF_p4	TTGAGCCCATTTCGCAAAAC	GGGCAAGTTAGCGTCTGAAT	45.0	50.0	57.9	58.3	1728 bp	0.333333	50.1
psaF_p5	TGATCGACGATCTGTTGAGC	GGGCAAGTTAGCGTCTGAAT	50.0	50.0	57.8	58.3	1665 bp	0.333333	49.7