Hotspot

psbY-rrf

IRa · 114257-114562 · 306 bp

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Pi 0.153
MarkerSeek score 82.70
Primer available yes
Label psbY-rrf

Feature Metadata

label_name
psbY-rrf
feature_type
igs
region
IRa
length_bp
306
variable_sites
63
pi
0.153
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbY-rrf_p1 ATAATGCCCATGAAGCAGCA AACCACACTGATCCATTCCG 45.0 50.0 57.9 57.9 454-465 bp 1.0 90.5
psbY-rrf_p2 ATAATGCCCATGAAGCAGCA CGGAACCACACTGATCCATT 45.0 50.0 57.9 57.9 457-468 bp 1.0 90.5
psbY-rrf_p3 TAATGCCCATGAAGCAGCAA AACCACACTGATCCATTCCG 45.0 50.0 58.2 57.9 453-464 bp 1.0 89.9
psbY-rrf_p4 TGCCCATGAAGCAGCAATTA AACCACACTGATCCATTCCG 45.0 50.0 58.2 57.9 450-461 bp 1.0 89.9
psbY-rrf_p5 TGCCCATGAAGCAGCAATTA CGGAACCACACTGATCCATT 45.0 50.0 58.2 57.9 453-464 bp 1.0 89.9