Hotspot

clpC-psbA

SSC · 104081-105189 · 1109 bp

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Pi 0.451
MarkerSeek score 79.40
Primer available yes
Label clpC-psbA

Feature Metadata

label_name
clpC-psbA
feature_type
igs
region
SSC
length_bp
1109
variable_sites
560
pi
0.451
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpC-psbA_p1 AACCGCTTCCAGAACCAATT TTCTCAGCTATGCACGGTTC 45.0 50.0 58.0 58.0 1687-2209 bp 0.666667 78.5
clpC-psbA_p2 AACCGCTTCCAGAACCAATT ATTCCAAGCTGAGCACAACA 45.0 45.0 58.0 58.0 1763-2285 bp 0.666667 78.5
clpC-psbA_p3 AACCGCTTCCAGAACCAATT TCCAAGCTGAGCACAACATT 45.0 45.0 58.0 58.0 1761-2283 bp 0.666667 78.5
clpC-psbA_p4 AACCGCTTCCAGAACCAATT TTCCAAGCTGAGCACAACAT 45.0 45.0 58.0 58.0 1762-2284 bp 0.666667 78.5
clpC-psbA_p5 GGCTGAGCAGTGTTTATCGA TTCTCAGCTATGCACGGTTC 50.0 50.0 58.0 58.0 1887 bp 0.333333 53.2