Hotspot

OOD24_pgt025-OOD24_pgt026

SSC · 101011-101107 · 97 bp

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Pi 0.236
MarkerSeek score 82.70
Primer available yes
Label OOD24_pgt025-OOD24_pgt026

Feature Metadata

label_name
OOD24_pgt025-OOD24_pgt026
feature_type
igs
region
SSC
length_bp
97
variable_sites
33
pi
0.236
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
OOD24_pgt025-OOD24_pgt026_p1 CTTGAACCCTCACGCCTATC GGTATGGCCAAGTGGTAAGG 55.0 55.0 58.1 57.9 233-237 bp 1.0 88.5
OOD24_pgt025-OOD24_pgt026_p2 AACCCTCACGCCTATCACTA GGTATGGCCAAGTGGTAAGG 50.0 55.0 57.8 57.9 229-233 bp 1.0 87.7
OOD24_pgt025-OOD24_pgt026_p3 TAAGTCCGTCGTGTCTACCA GGTATGGCCAAGTGGTAAGG 50.0 55.0 57.8 57.9 197-201 bp 1.0 87.7
OOD24_pgt025-OOD24_pgt026_p4 ACCCTCACGCCTATCACTAG GGTATGGCCAAGTGGTAAGG 55.0 55.0 58.3 57.9 228-232 bp 1.0 86.5
OOD24_pgt025-OOD24_pgt026_p5 CTTGAACCCTCACGCCTATC CGGTATGGCCAAGTGGTAAG 55.0 55.0 58.1 58.3 234-238 bp 1.0 86.2