Hotspot

thiG-ycf35

SSC · 75003-76139 · 1137 bp

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Pi 0.425
MarkerSeek score 78.90
Primer available yes
Label thiG-ycf35

Feature Metadata

label_name
thiG-ycf35
feature_type
igs
region
SSC
length_bp
1137
variable_sites
554
pi
0.425
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
thiG-ycf35_p1 CCAGCACAAATGGCTAAAGC GCAACCCTACCCAGTTGAAA 50.0 50.0 58.0 58.0 870-1435 bp 0.666667 78.6
thiG-ycf35_p2 ATACAGCAGGTGCACAAACA GCAACCCTACCCAGTTGAAA 45.0 50.0 58.0 58.0 1322-1887 bp 0.666667 78.4
thiG-ycf35_p3 ACCCAGTGAAGCAACTCTTG GCAACCCTACCCAGTTGAAA 50.0 50.0 58.0 58.0 1514 bp 0.333333 53.0
thiG-ycf35_p4 CACCCAGTGAAGCAACTCTT GCAACCCTACCCAGTTGAAA 50.0 50.0 58.0 58.0 1515 bp 0.333333 53.0
thiG-ycf35_p5 TATGGAAATTGGTGCCGAGG GCAACCCTACCCAGTTGAAA 50.0 50.0 57.9 58.0 1493 bp 0.333333 52.8