Hotspot

OOD24_pgt007-OOD24_pgt008

LSC · 23231-23366 · 136 bp

Back to Haslea

Pi 0.145
MarkerSeek score 80.20
Primer available yes
Label OOD24_pgt007-OOD24_pgt008

Feature Metadata

label_name
OOD24_pgt007-OOD24_pgt008
feature_type
igs
region
LSC
length_bp
136
variable_sites
28
pi
0.145
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
OOD24_pgt007-OOD24_pgt008_p1 AACCTGTGACCTTCTGCTTG AAAATGGGCCGAGTTGGATT 50.0 45.0 58.0 58.1 276 bp 1.0 87.9
OOD24_pgt007-OOD24_pgt008_p2 AACCTGTGACCTTCTGCTTG GATTTACAATCCGCCCCCTT 50.0 50.0 58.0 57.9 231 bp 1.0 87.9
OOD24_pgt007-OOD24_pgt008_p3 TTGAACCTGTGACCTTCTGC GATTTACAATCCGCCCCCTT 50.0 50.0 58.0 57.9 234 bp 1.0 87.9
OOD24_pgt007-OOD24_pgt008_p4 TTGAACCTGTGACCTTCTGC AAAATGGGCCGAGTTGGATT 50.0 45.0 58.0 58.1 279 bp 1.0 87.8
OOD24_pgt007-OOD24_pgt008_p5 AGACACTCTACCGCTGAGTT AAAATGGGCCGAGTTGGATT 50.0 45.0 58.1 58.1 250 bp 1.0 87.4