Hotspot

OOD24_pgt005-orf125a

LSC · 21284-22409 · 1126 bp

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Pi 0.309
MarkerSeek score 65.70
Primer available yes
Label OOD24_pgt005-orf125a

Feature Metadata

label_name
OOD24_pgt005-orf125a
feature_type
igs
region
LSC
length_bp
1126
variable_sites
81
pi
0.309
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.20
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
OOD24_pgt005-orf125a_p1 ATCAGTGTAAGTGCTGCAGG TTGCTGGCTTTCCTTGTGAT 50.0 45.0 57.9 58.0 1503 bp 0.333333 52.5
OOD24_pgt005-orf125a_p2 ATCAGTGTAAGTGCTGCAGG TCGCTTACCACTATCGGCTA 50.0 50.0 57.9 58.0 1592 bp 0.333333 52.3
OOD24_pgt005-orf125a_p3 ATCAGTGTAAGTGCTGCAGG GAAGATGGATCTGCTGCACA 50.0 50.0 57.9 58.0 1804 bp 0.333333 52.2
OOD24_pgt005-orf125a_p4 ATCAGTGTAAGTGCTGCAGG GTGAAGATGGATCTGCTGCA 50.0 50.0 57.9 58.0 1806 bp 0.333333 52.2
OOD24_pgt005-orf125a_p5 ATCAGTGTAAGTGCTGCAGG TGAAGATGGATCTGCTGCAC 50.0 50.0 57.9 58.0 1805 bp 0.333333 52.2