Hotspot

rpl20

LSC · 71266-71619 · 354 bp

Back to Haplophyllum

Pi 0.0113

MarkerSeek score 45.40

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 354
variable_sites: 4
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	TGAAAGAAGTGGGTTGACCG	GAGCCGAAGAGTGGTAACTG	50.0	55.0	58.0	58.0	1021 bp	1.0	82.8
rpl20_p2	TGAAAGAAGTGGGTTGACCG	GACGGAACCCACAGTTTCTT	50.0	50.0	58.0	58.0	913 bp	1.0	82.7
rpl20_p3	TGAAAGAAGTGGGTTGACCG	CAGCTACCCCACCAATCTTC	50.0	55.0	58.0	58.0	1112 bp	1.0	82.3
rpl20_p4	TGAAAGAAGTGGGTTGACCG	TCCATTACGCGGGGTAAATG	50.0	50.0	58.0	58.0	1188 bp	1.0	82.1
rpl20_p5	TGAAAGAAGTGGGTTGACCG	TGGCTCCGGTGTATAGAGAG	50.0	55.0	58.0	58.0	965 bp	0.5	59.6