Hotspot

rpl32-trnL-UAG

SSC · 116711-117466 · 756 bp

Back to Haplophyllum

Pi 0.00928
MarkerSeek score 56.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
756
variable_sites
7
pi
0.00928
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGTTGAAGGCTTTTTCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 869-880 bp 1.0 79.4
rpl32-trnL-UAG_p2 GCGTTGAAGGCTTTTTCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 870-881 bp 1.0 79.4
rpl32-trnL-UAG_p3 TTGAAGGCTTTTTCGTTGGC AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 866-877 bp 1.0 79.0
rpl32-trnL-UAG_p4 TTGAAGGCTTTTTCGTTGGC CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 867-878 bp 1.0 79.0
rpl32-trnL-UAG_p5 GCGTTGAAGGCTTTTTCGTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 879-890 bp 1.0 78.5