Hotspot

psbC-trnS-UGA

LSC · 36785-37038 · 254 bp

Back to Haplophyllum

Pi 0.0363

MarkerSeek score 75.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 254
variable_sites: 9
pi: 0.0363
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	398-403 bp	1.0	79.0
psbC-trnS-UGA_p2	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	433-438 bp	1.0	78.9
psbC-trnS-UGA_p3	GCTGCAGCAGGATTTGAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	344-349 bp	1.0	78.2
psbC-trnS-UGA_p4	TAGGTCATTTATGGCACGCG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.4	58.2	379-384 bp	1.0	78.0
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	TGGTTGATAGCTCCGGTCTT	50.0	50.0	58.4	58.4	434-439 bp	1.0	75.9