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petN-psbM

LSC · 29025-30212 · 1188 bp

Back to Haplophyllum

Pi 0.0111

MarkerSeek score 66.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1188
variable_sites: 13
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	CGTTCTAGTTCCTACTGCGT	45.0	50.0	58.0	57.1	1328-1578 bp	1.0	74.1
petN-psbM_p2	ACATTTTCCCTTTCGCTCGT	CGTTCTAGTTCCTACTGCGT	45.0	50.0	58.1	57.1	1297-1547 bp	1.0	73.3
petN-psbM_p3	CCCTTTCGCTCGTAGTATGG	CGTTCTAGTTCCTACTGCGT	55.0	50.0	57.9	57.1	1290-1540 bp	1.0	73.1
petN-psbM_p4	CCTTTCGCTCGTAGTATGGG	CGTTCTAGTTCCTACTGCGT	55.0	50.0	57.9	57.1	1289-1539 bp	1.0	73.1
petN-psbM_p5	CTTTCGCTCGTAGTATGGGG	CGTTCTAGTTCCTACTGCGT	55.0	50.0	57.9	57.1	1288-1538 bp	1.0	73.1