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trnH-GUG-psbA

LSC · 76-541 · 466 bp

Back to Haplophyllum

Pi 0.0558

MarkerSeek score 71.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 466
variable_sites: 26
pi: 0.0558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCACTATGGCGTTCAACCTA	55.0	50.0	58.1	58.0	679-685 bp	1.0	82.1
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TAGCACTATGGCGTTCAACC	55.0	50.0	58.1	58.0	681-687 bp	1.0	82.1
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	CATTTCTTCTTGGCGGCTTG	55.0	50.0	58.1	58.0	737-743 bp	1.0	81.9
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	CATTTCTTCTTGGCGGCTTG	50.0	50.0	57.9	58.0	751-757 bp	1.0	81.8
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	CATTTCTTCTTGGCGGCTTG	50.0	50.0	57.9	58.0	750-756 bp	1.0	81.8