Hotspot

ndhG-ndhI

SSC · 124088-124498 · 411 bp

Back to Hansenia

Pi 0.0296

MarkerSeek score 68.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 411
variable_sites: 28
pi: 0.0296
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GCAATCGCACCTATTAACGC	TTCTTTGGGGCGTTTACCAA	50.0	45.0	58.0	57.9	1000-1069 bp	1.0	84.4
ndhG-ndhI_p2	GCAATCGCACCTATTAACGC	TTTCTTTGGGGCGTTTACCA	50.0	45.0	58.0	57.9	1001-1070 bp	1.0	84.4
ndhG-ndhI_p3	CATTTATGGCCCCCACGTAA	ACAAACAGTACGAGCTGCAA	50.0	45.0	57.9	58.1	1080-1149 bp	1.0	83.8
ndhG-ndhI_p4	GCAATCGCACCTATTAACGC	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	1382-1451 bp	1.0	83.2
ndhG-ndhI_p5	GCAATCGCACCTATTAACGC	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	1381-1450 bp	1.0	83.2