Hotspot

petA-psbJ

LSC · 67283-68262 · 980 bp

Back to Hansenia

Pi 0.0119

MarkerSeek score 63.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 26
pi: 0.0119
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTATTTTGGCACA	GCAGGTATTCTTGTGATCGGT	42.9	47.6	57.4	58.1	1047-1217 bp	1.0	64.4
petA-psbJ_p2	TGGCATCTGTTATTTTGGCAC	GCAGGTATTCTTGTGATCGGT	42.9	47.6	57.4	58.1	1048-1218 bp	1.0	64.4
petA-psbJ_p3	GGCATCTGTTATTTTGGCACA	AGCAGGTATTCTTGTGATCGG	42.9	47.6	57.4	57.8	1048-1218 bp	1.0	63.7
petA-psbJ_p4	TGGCATCTGTTATTTTGGCAC	AGCAGGTATTCTTGTGATCGG	42.9	47.6	57.4	57.8	1049-1219 bp	1.0	63.7
petA-psbJ_p5	TTGGCATCTGTTATTTTGGCAC	GCAGGTATTCTTGTGATCGGT	40.9	47.6	58.1	58.1	1049-1219 bp	1.0	60.5