Hotspot

ycf4-cemA

LSC · 64540-65382 · 843 bp

Back to Hansenia

Pi 0.0134

MarkerSeek score 69.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 30
pi: 0.0134
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AATCGAACAAAAAGCTGCCG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.9	58.1	865-998 bp	1.0	85.5
ycf4-cemA_p2	CGAACAAAAAGCTGCCGAAT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.9	58.1	862-995 bp	1.0	85.5
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	843-976 bp	1.0	85.2
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	859-992 bp	1.0	84.7
ycf4-cemA_p5	TTGCGTGTACCGATTGAAGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	831-964 bp	1.0	84.7