Hotspot

accD-psaI

LSC · 62946-63435 · 490 bp

Back to Hansenia

Pi 0.0105

MarkerSeek score 62.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 17
pi: 0.0105
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCACGCTTTCTTTCCTTTGA	TGGACTTATTCATCTTTCTACATGA	42.9	32.0	58.1	55.8	570-584 bp	0.857143	36.7
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	TGGACTTATTCATCTTTCTACATGA	50.0	32.0	58.8	55.8	572-586 bp	0.857143	36.6
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	TGGACTTATTCATCTTTCTACATGA	52.6	32.0	58.1	55.8	572-586 bp	0.857143	36.6
accD-psaI_p4	GCTCCACGCTTTCTTTCCTT	TCTAAGTGAGTATAGAATGGACT	50.0	34.8	58.8	53.5	589-603 bp	0.857143	36.6
accD-psaI_p5	GAGTTATTTCAGCTCCACGC	TGGACTTATTCATCTTTCTACATGA	50.0	32.0	56.9	55.8	583-597 bp	0.857143	36.6