Hotspot

trnE-UUC-trnT-GGU

LSC · 34319-35051 · 733 bp

Back to Hansenia

Pi 0.0586

MarkerSeek score 86.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 65
pi: 0.0586
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	688-861 bp	1.0	89.4
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	653-826 bp	1.0	88.9
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	685-858 bp	1.0	88.2
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	690-863 bp	1.0	87.1
trnE-UUC-trnT-GGU_p5	TTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	57.4	58.0	723-896 bp	1.0	86.0