Hotspot

petN-psbM

LSC · 31897-33085 · 1189 bp

Back to Hansenia

Pi 0.0274

MarkerSeek score 57.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1189
variable_sites: 45
pi: 0.0274
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTCCCTTTTTGACTCTGCG	AGTATCCCCTTCTGACTCCG	50.0	55.0	58.1	57.9	1344-1859 bp	1.0	85.0
petN-psbM_p2	AAAGGGATTGTGCATTCGGT	AGTATCCCCTTCTGACTCCG	45.0	55.0	58.1	57.9	1864-2381 bp	1.0	84.9
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGTATCCCCTTCTGACTCCG	50.0	55.0	58.2	57.9	1246-1761 bp	1.0	84.8
petN-psbM_p4	TCCACGGTAGCATAAAGGGA	AGTATCCCCTTCTGACTCCG	50.0	55.0	58.1	57.9	1877-2394 bp	1.0	84.5
petN-psbM_p5	CCGTTGGATAGACAGACACG	AGTATCCCCTTCTGACTCCG	55.0	55.0	58.1	57.9	2095-2219 bp	0.428571	61.0