Hotspot

ycf1

SSC · 128984-134530 · 5547 bp

Back to Hamamelis

Pi 0.000961

MarkerSeek score 26.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5547
variable_sites: 8
pi: 0.000961
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TATCCCAGGCTTCCGCTATT	CGCGCTCAAAGACGTAAAAC	50.0	50.0	58.3	58.4	280 bp	1.0	75.0
ycf1_p2	ACAATGCTATCCCAGGCTTC	CGCGCTCAAAGACGTAAAAC	50.0	50.0	57.6	58.4	287 bp	1.0	74.3
ycf1_p3	CAATGCTATCCCAGGCTTCC	CGCGCTCAAAGACGTAAAAC	55.0	50.0	58.4	58.4	286 bp	1.0	74.1
ycf1_p4	TATCCCAGGCTTCCGCTATT	GCGCTCAAAGACGTAAAACA	50.0	45.0	58.3	57.3	279 bp	1.0	73.2
ycf1_p5	ACAATGCTATCCCAGGCTTC	GCGCTCAAAGACGTAAAACA	50.0	45.0	57.6	57.3	286 bp	1.0	72.5