Hotspot

rpoC1

LSC · 22253-25047 · 2795 bp

Back to Hamamelis

Pi 0.000477

MarkerSeek score 56.40

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2795
variable_sites: 2
pi: 0.000477
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GAGAGGACCGAATTCACACC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.0	58.0	2969 bp	1.0	79.6
rpoC1_p2	GGAGAGGACCGAATTCACAC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.0	58.0	2970 bp	1.0	79.6
rpoC1_p3	CCTAGTTGAACCGGAGAGGA	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.2	58.0	2982 bp	1.0	78.5
rpoC1_p4	GGACCGAATTCACACCGTAT	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.7	58.0	2965 bp	1.0	77.3
rpoC1_p5	GAGAGGACCGAATTCACACC	GCTCGTTCGAGAACTACGAT	55.0	50.0	58.0	57.5	2967 bp	1.0	76.3