Hotspot

ndhI

SSC · 124292-124873 · 582 bp

Back to Hamamelis

Pi 0.00229

MarkerSeek score 59.80

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 582
variable_sites: 2
pi: 0.00229
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TGGTAATTTTTCTTGAATCAAGGGG	CTTCCCAACTCCTTTCACTGT	36.0	47.6	58.0	57.8	631 bp	1.0	40.3
ndhI_p2	GGTAATTTTTCTTGAATCAAGGGGT	CTTCCCAACTCCTTTCACTGT	36.0	47.6	58.0	57.8	630 bp	1.0	40.3
ndhI_p3	TGGTAATTTTTCTTGAATCAAGGGG	ACCTAGGCTAAGAATGGACCA	36.0	47.6	58.0	57.9	717 bp	1.0	40.2
ndhI_p4	GGTAATTTTTCTTGAATCAAGGGGT	ACCTAGGCTAAGAATGGACCA	36.0	47.6	58.0	57.9	716 bp	1.0	40.2
ndhI_p5	TTTTTCTTGAATCAAGGGGT	ACCTAGGCTAAGAATGGACCA	35.0	47.6	52.8	57.9	711 bp	1.0	40.2