Hotspot

clpP

LSC · 74028-76078 · 2051 bp

Back to Hamamelis

Pi 0.000651

MarkerSeek score 58.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2051
variable_sites: 2
pi: 0.000651
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GATTTGCGTGAAATCCCTGC	TCCATTGTTACGTTTCCAAATCA	50.0	34.8	58.1	57.0	2136-2138 bp	1.0	47.3
clpP_p2	TTTGCGTGAAATCCCTGCTA	TCCATTGTTACGTTTCCAAATCA	45.0	34.8	57.8	57.0	2134-2136 bp	1.0	46.4
clpP_p3	GATTTGCGTGAAATCCCTGC	ACGTTTCCAAATCAAAGTGA	50.0	35.0	58.1	53.6	2127-2129 bp	1.0	44.7
clpP_p4	TGCGTGAAATCCCTGCTATT	TCCATTGTTACGTTTCCAAATCA	45.0	34.8	57.6	57.0	2132-2134 bp	1.0	44.6
clpP_p5	TTGCGTGAAATCCCTGCTAT	TCCATTGTTACGTTTCCAAATCA	45.0	34.8	57.6	57.0	2133-2135 bp	1.0	44.6