Hotspot

trnL-CAA-ycf15

IRa · 149778-150406 · 629 bp

Back to Hamamelis

Pi 0.00217

MarkerSeek score 49.70

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 629
variable_sites: 2
pi: 0.00217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	GGTTGTTGCTATCTGCTCCA	50.0	50.0	58.0	57.9	728-742 bp	1.0	80.8
trnL-CAA-ycf15_p2	AAATGGTAGACACGCGAGAC	TGGTTGTTGCTATCTGCTCC	50.0	50.0	58.0	57.9	729-743 bp	1.0	80.8
trnL-CAA-ycf15_p3	TGAAATGGTAGACACGCGAG	GGTTGTTGCTATCTGCTCCA	50.0	50.0	58.0	57.9	730-744 bp	1.0	80.8
trnL-CAA-ycf15_p4	TGAAATGGTAGACACGCGAG	TGGTTGTTGCTATCTGCTCC	50.0	50.0	58.0	57.9	731-745 bp	1.0	80.8
trnL-CAA-ycf15_p5	GCCTTGGTGGTGAAATGGTA	GGTTGTTGCTATCTGCTCCA	50.0	50.0	58.1	57.9	740-754 bp	1.0	80.2