Hotspot

ndhD-psaC

SSC · 122149-122335 · 187 bp

Back to Hamamelis

Pi 0

MarkerSeek score 49.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 187
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AAAATTAAGGAACCCGCGGA	AGAGGACTGTGTCGGTTGTA	45.0	50.0	57.8	58.0	309-364 bp	1.0	82.3
ndhD-psaC_p2	AAATTAAGGAACCCGCGGAT	AGAGGACTGTGTCGGTTGTA	45.0	50.0	57.6	58.0	308-363 bp	1.0	80.5
ndhD-psaC_p3	CGCGGATATCGGCAAAACTA	AGAGGACTGTGTCGGTTGTA	50.0	50.0	58.5	58.0	295-350 bp	1.0	79.9
ndhD-psaC_p4	AAAATTAAGGAACCCGCGGA	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.8	57.7	290-345 bp	1.0	79.8
ndhD-psaC_p5	GAAAATTAAGGAACCCGCGG	AGAGGACTGTGTCGGTTGTA	50.0	50.0	57.4	58.0	310-365 bp	1.0	79.5