Hotspot

ycf15-trnL-CAA

IRb · 97764-98392 · 629 bp

Back to Hamamelis

Pi 0.00217
MarkerSeek score 49.70
Primer available yes
Label ycf15-trnL-CAA

Feature Metadata

label_name
ycf15-trnL-CAA
feature_type
igs
region
IRb
length_bp
629
variable_sites
2
pi
0.00217
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnL-CAA_p1 GGTTGTTGCTATCTGCTCCA AAATGGTAGACACGCGAGAC 50.0 50.0 57.9 58.0 728-742 bp 1.0 80.8
ycf15-trnL-CAA_p2 TGGTTGTTGCTATCTGCTCC AAATGGTAGACACGCGAGAC 50.0 50.0 57.9 58.0 729-743 bp 1.0 80.8
ycf15-trnL-CAA_p3 GGTTGTTGCTATCTGCTCCA TGAAATGGTAGACACGCGAG 50.0 50.0 57.9 58.0 730-744 bp 1.0 80.8
ycf15-trnL-CAA_p4 TGGTTGTTGCTATCTGCTCC TGAAATGGTAGACACGCGAG 50.0 50.0 57.9 58.0 731-745 bp 1.0 80.8
ycf15-trnL-CAA_p5 GGTTGTTGCTATCTGCTCCA GCCTTGGTGGTGAAATGGTA 50.0 50.0 57.9 58.1 740-754 bp 1.0 80.2