Hotspot

trnF-GAA-ndhJ

LSC · 51620-52345 · 726 bp

Back to Hamamelis

Pi 0.000918

MarkerSeek score 47.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 1
pi: 0.000918
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	866-868 bp	1.0	80.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	830-832 bp	1.0	79.7
trnF-GAA-ndhJ_p3	TGAGGATGATACGTCGGGAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.9	58.1	891-893 bp	1.0	79.5
trnF-GAA-ndhJ_p4	GGATGATACGTCGGGAATGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	888-890 bp	1.0	79.4
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	ACGCCTGAAACGCATCTTAA	55.0	45.0	58.0	58.2	894-896 bp	1.0	79.1