Hotspot

psbZ-trnG-GCC

LSC · 39230-39854 · 625 bp

Back to Hamamelis

Pi 0.00213
MarkerSeek score 48.20
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
625
variable_sites
2
pi
0.00213
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 780-782 bp 1.0 77.5
psbZ-trnG-GCC_p2 GCTTCTCCTGATGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.3 57.8 778-780 bp 1.0 77.1
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG TACCATTCGACCATATCCGC 50.0 50.0 57.8 57.3 753-755 bp 1.0 73.0
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC TACCATTCGACCATATCCGC 55.0 50.0 58.3 57.3 751-753 bp 1.0 72.6
psbZ-trnG-GCC_p5 CTCCTGATGGTTGGTCAAGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.1 57.8 774-776 bp 1.0 72.2