Hotspot

rps18

LSC · 68015-68320 · 306 bp

Back to Haloxylon

Pi 0.00327

MarkerSeek score 54.20

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 306
variable_sites: 1
pi: 0.00327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	ACAAATCCTATTTAGGCTGGACC	GAACAAAACCGCAATCCGTT	43.5	45.0	58.2	57.9	450 bp	1.0	53.5
rps18_p2	AGGATTTTCGGTATAAGGACGA	GAACAAAACCGCAATCCGTT	40.9	45.0	56.7	57.9	395 bp	1.0	52.9
rps18_p3	CAAATCCTATTTAGGCTGGACC	GAACAAAACCGCAATCCGTT	45.5	45.0	56.5	57.9	449 bp	1.0	51.5
rps18_p4	ACAAATCCTATTTAGGCTGGACC	TTCGAACAAAACCGCAATCC	43.5	45.0	58.2	57.6	453 bp	1.0	51.2
rps18_p5	AGGATTTTCGGTATAAGGACGA	TTCGAACAAAACCGCAATCC	40.9	45.0	56.7	57.6	398 bp	1.0	50.7