Hotspot

rps16

LSC · 4870-5985 · 1116 bp

Back to Haloxylon

Pi 0.000896

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1116
variable_sites: 1
pi: 0.000896
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACAGGAATAGAAAGGGAAGA	CTGTGTATTGACTGTCCCGG	40.0	55.0	52.9	58.0	1308 bp	1.0	40.1
rps16_p2	TCTACAGGAATAGAAAGGGAAGA	CTGTGTATTGACTGTCCCGG	39.1	55.0	55.8	58.0	1311 bp	1.0	40.1
rps16_p3	ACAGGAATAGAAAGGGAAGAA	CTGTGTATTGACTGTCCCGG	38.1	55.0	53.8	58.0	1308 bp	1.0	40.1
rps16_p4	ACAGGAATAGAAAGGGAAGAAA	CTGTGTATTGACTGTCCCGG	36.4	55.0	54.7	58.0	1308 bp	1.0	40.1
rps16_p5	ACAGGAATAGAAAGGGAAGA	CGGCGGAACCAATGACTATT	40.0	50.0	52.9	58.3	1291 bp	1.0	40.1