Hotspot

rpoC2

LSC · 16170-20285 · 4116 bp

Back to Haloxylon

Pi 0.000729

MarkerSeek score 54.30

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4116
variable_sites: 3
pi: 0.000729
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCCTTGAATCGGAATGGACC	GCGCTTCCTTTGTTGAAGTC	55.0	50.0	58.3	58.0	304 bp	1.0	77.4
rpoC2_p2	CCTTGAATCGGAATGGACCC	GCGCTTCCTTTGTTGAAGTC	55.0	50.0	58.3	58.0	303 bp	1.0	77.4
rpoC2_p3	CTTGAATCGGAATGGACCCA	GCGCTTCCTTTGTTGAAGTC	50.0	50.0	57.6	58.0	302 bp	1.0	76.6
rpoC2_p4	ACCCTTGAATCGGAATGGAC	GCGCTTCCTTTGTTGAAGTC	50.0	50.0	57.6	58.0	305 bp	1.0	76.6
rpoC2_p5	CCTTGAATCGGAATGGACCC	TTCTATTAAACAGGCCCGCG	55.0	50.0	58.3	58.3	320 bp	1.0	75.0