Hotspot

rpoA

LSC · 78004-79014 · 1011 bp

Back to Haloxylon

Pi 0.00198

MarkerSeek score 59.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1011
variable_sites: 2
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	ACGATGTGTGTATCTAGGGA	CCACATAATGGCTGTAGGC	45.0	52.6	55.1	55.7	1219 bp	1.0	40.1
rpoA_p2	ACGATGTGTGTATCTAGGGAA	CCACATAATGGCTGTAGGC	42.9	52.6	55.9	55.7	1219 bp	1.0	40.1
rpoA_p3	TGTGTATCTAGGGAATAGTCGC	CCACATAATGGCTGTAGGC	45.5	52.6	56.9	55.7	1213 bp	1.0	40.1
rpoA_p4	GTGTGTATCTAGGGAATAGTCGC	CCACATAATGGCTGTAGGC	47.8	52.6	58.2	55.7	1214 bp	1.0	40.1
rpoA_p5	ACGATGTGTGTATCTAGGGA	AGACGCGTGTAGAAATAAGA	45.0	40.0	55.1	54.3	1189 bp	1.0	40.1