Hotspot

rpl16

LSC · 81282-82600 · 1319 bp

Back to Haloxylon

Pi 0.00303

MarkerSeek score 50.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1319
variable_sites: 4
pi: 0.00303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAATTGTTATCCAAAAGATCCA	TGACCCGTTGATAGAATTGCT	33.3	42.9	56.6	57.4	1379 bp	1.0	40.2
rpl16_p2	TGTTATCCAAAAGATCCATTTCCT	TGACCCGTTGATAGAATTGCT	33.3	42.9	56.3	57.4	1373 bp	1.0	40.2
rpl16_p3	TCCAATTGTTATCCAAAAGATCCA	TCGATTGACCCGTTGATAGA	33.3	45.0	56.6	56.1	1384 bp	1.0	40.2
rpl16_p4	TCCAAAAGATCCATTTCCTT	TGACCCGTTGATAGAATTGCT	35.0	42.9	52.3	57.4	1368 bp	1.0	40.2
rpl16_p5	TCCAAAAGATCCATTTCCTTTAGT	TGACCCGTTGATAGAATTGCT	33.3	42.9	56.3	57.4	1368 bp	1.0	40.2