Hotspot

atpI

LSC · 14264-15007 · 744 bp

Back to Haloxylon

Pi 0.00134
MarkerSeek score 59.50
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
744
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 GAGTATCTTGAGCCACACAT AGGTCGAGGAAGAGATGGTT 45.0 50.0 54.9 57.8 876 bp 1.0 53.1
atpI_p2 GAGTATCTTGAGCCACACAT GGTCGAGGAAGAGATGGTTG 45.0 55.0 54.9 57.7 875 bp 1.0 52.6
atpI_p3 TGAGCCACACATATATTACCTGG AGGTCGAGGAAGAGATGGTT 43.5 50.0 57.8 57.8 868 bp 1.0 52.4
atpI_p4 TGAGCCACACATATATTACCTGG GGTCGAGGAAGAGATGGTTG 43.5 55.0 57.8 57.7 867 bp 1.0 51.9
atpI_p5 GAGTATCTTGAGCCACACAT CAGGTCGAGGAAGAGATGGT 45.0 55.0 54.9 58.5 877 bp 1.0 50.8