Hotspot

ndhF-rpl32

SSC · 110959-112062 · 1104 bp

Back to Haloxylon

Pi 0.000906

MarkerSeek score 59.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1104
variable_sites: 1
pi: 0.000906
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAACGGGAAGTGTAAGGA	AAAAGGCTTTTAACGCTGCC	50.0	45.0	58.0	57.9	1258 bp	1.0	78.7
ndhF-rpl32_p2	AGGAACGGGAAGTGTAAGGA	TTTAACGCTGCCCAATACCC	50.0	50.0	58.0	58.5	1250 bp	1.0	75.6
ndhF-rpl32_p3	AACAAAGGAACGGGAAGTGT	AAAAGGCTTTTAACGCTGCC	45.0	45.0	57.6	57.9	1263 bp	1.0	75.4
ndhF-rpl32_p4	AGGAACGGGAAGTGTAAGGA	GCTGCCCAATACCCCTTTTT	50.0	50.0	58.0	58.7	1244 bp	1.0	74.1
ndhF-rpl32_p5	AGGAACGGGAAGTGTAAGGA	GCTTTTAACGCTGCCCAATA	50.0	45.0	58.0	57.1	1253 bp	1.0	72.5