Hotspot

petA-psbJ

LSC · 63054-63867 · 814 bp

Back to Haloxylon

Pi 0.00983

MarkerSeek score 64.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 814
variable_sites: 8
pi: 0.00983
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CACTGCGTGTACAAGGTCTT	CTGGATTAGGCTCATCGCTG	50.0	55.0	58.1	58.2	953 bp	1.0	77.9
petA-psbJ_p2	CACTGCGTGTACAAGGTCTT	TCTGGATTAGGCTCATCGCT	50.0	50.0	58.1	58.3	954 bp	1.0	77.3
petA-psbJ_p3	CACTGCGTGTACAAGGTCTT	TTCTGGATTAGGCTCATCGC	50.0	50.0	58.1	57.5	955 bp	1.0	75.3
petA-psbJ_p4	CGAGAAGGTTCAACTGTCCG	CTGGATTAGGCTCATCGCTG	55.0	55.0	58.6	58.2	871 bp	1.0	74.4
petA-psbJ_p5	ACTGCGTGTACAAGGTCTTT	CTGGATTAGGCTCATCGCTG	45.0	55.0	57.4	58.2	952 bp	1.0	74.1