Hotspot

psbM-trnD-GUC

LSC · 29078-30231 · 1154 bp

Back to Haloxylon

Pi 0.000867
MarkerSeek score 59.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1154
variable_sites
1
pi
0.000867
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TTTGGCTGACCGTTTTTACA TCAATTGGTTAGAGCACCGC 40.0 50.0 56.1 58.6 1314 bp 1.0 60.4
psbM-trnD-GUC_p2 TTGGCTGACCGTTTTTACAT TCAATTGGTTAGAGCACCGC 40.0 50.0 55.9 58.6 1313 bp 1.0 58.5
psbM-trnD-GUC_p3 TTTTGGCTGACCGTTTTTACA TCAATTGGTTAGAGCACCGC 38.1 50.0 56.9 58.6 1315 bp 1.0 58.5
psbM-trnD-GUC_p4 TGGCTGACCGTTTTTACATAGA TCAATTGGTTAGAGCACCGC 40.9 50.0 57.7 58.6 1312 bp 1.0 57.5
psbM-trnD-GUC_p5 TTTGGCTGACCGTTTTTACAT TCAATTGGTTAGAGCACCGC 38.1 50.0 56.6 58.6 1314 bp 1.0 56.7