Hotspot

rpoB-trnC-GCA

LSC · 26515-27630 · 1116 bp

Back to Haloxylon

Pi 0.0018

MarkerSeek score 60.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 2
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCCATCCCGTAGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.1	58.1	1200 bp	1.0	78.9
rpoB-trnC-GCA_p2	CCTCATTTCCATCCCGTAGC	AAAGGATTTGCAGTCCTCCG	55.0	50.0	58.1	58.2	1181 bp	1.0	78.2
rpoB-trnC-GCA_p3	CATCCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.7	58.1	1205 bp	1.0	77.4
rpoB-trnC-GCA_p4	CATCCCCTCATTTCCATCCC	AAAGGATTTGCAGTCCTCCG	55.0	50.0	57.7	58.2	1186 bp	1.0	76.7
rpoB-trnC-GCA_p5	CCCTCATTTCCATCCCGTAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.4	58.1	1201 bp	1.0	75.4