Hotspot

atpH-atpI

LSC · 13576-14263 · 688 bp

Back to Haloxylon

Pi 0.00146

MarkerSeek score 51.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 1
pi: 0.00146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.1	57.9	768-773 bp	1.0	78.7
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	826-831 bp	1.0	78.6
atpH-atpI_p3	AACGGAAGCAGCAGAAATCA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.1	57.8	738-743 bp	1.0	78.4
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	786-791 bp	1.0	78.4
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	796-801 bp	1.0	78.3