Hotspot

rpl16

LSC · 85784-87420 · 1637 bp

Back to Haloragis

Pi 0.00922

MarkerSeek score 56.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1637
variable_sites: 15
pi: 0.00922
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCCCAAGACCTCTTTTCTT	TCATTCAACATTTGGTATAATTGCT	45.0	28.0	55.9	55.8	1689-1690 bp	1.0	41.2
rpl16_p2	TCCCCAAGACCTCTTTTCTTTG	TCATTCAACATTTGGTATAATTGCT	45.5	28.0	58.2	55.8	1689-1690 bp	1.0	41.2
rpl16_p3	TCCCCAAGACCTCTTTTCTTT	TCATTCAACATTTGGTATAATTGCT	42.9	28.0	56.7	55.8	1689-1690 bp	1.0	41.2
rpl16_p4	ATCCCCAAGACCTCTTTTCT	TCATTCAACATTTGGTATAATTGCT	45.0	28.0	55.7	55.8	1690-1691 bp	1.0	41.2
rpl16_p5	CCCAAGACCTCTTTTCTTTGG	TCATTCAACATTTGGTATAATTGCT	47.6	28.0	56.7	55.8	1687-1688 bp	1.0	41.2